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Selection strategy and the design of hybrid oligonucleotide primers for RACE-PCR: cloning a family of toxin-like sequences from <Emphasis Type="Italic">Agelena orientalis</Emphasis> |
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| Authors: | Zhensheng Pan Richard Barry Alexey Lipkin Mikhail Soloviev |
| Institution: | (1) School of Biological Science, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UK;(2) Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya, 16/10, 117997, GSP, Moscow, V-437, Russia |
| Abstract: | Backgroundthe use of specific but partially degenerate primers for nucleic acid hybridisations and PCRs amplification of known or unknown gene families was first reported well over a decade ago and the technique has been used widely since then. |
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