PCR primers targeting <Emphasis Type="Italic">cis</Emphasis>-acting promoter elements in plants |
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Authors: | M Zhao Z Liu S Chen F Chen J Jiang A Song |
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Institution: | 1.College of Horticulture,Nanjing Agricultural University,Nanjing,China |
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Abstract: | The cis-acting elements present in gene promoters are important for promoter function. Thermal Asymmetric Interlaced PCR (TAIL-PCR)
provides a simple means for isolating promoter sequences, but the necessary primers can be troublesome to design. Here, we
describe an approach, which targets cis-acting elements for TAIL-PCR. The method combines the advantages of TAIL-PCR and SiteFinding-PCR. The new method proved successful
for isolating a number of plant promoter sequences. |
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Keywords: | |
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