MASTDISCS combi Carba plus,a simple method for discriminating carbapenemase‐producing Enterobacteriaceae,including OXA‐48‐type producers |
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| Authors: | Yusuke Ohsaki Ryoichi Kubo Jonathan Hobson Mya Davies Shunsuke Osaka Wataru Hayashi Yui Taniguchi Shota Koide Yukiko Nagano Noriyuki Nagano |
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| Affiliation: | 1. Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine, 3‐1‐1 Asahi, Matsumoto, Nagano 390‐8621, Japan;2. Kanto Chemical Co., Inc., East Muromachi Mitsui BLDG 2‐l, Nihombashi Muromachi 2‐chome, Chuo‐ku, Tokyo 103‐0022, Japan;3. Mast Group Ltd., Mast House, Derby Road, Bootle, Merseyside, L20 1EA, UK;4. School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, James Parsons Building, Liverpool, L3 3AF, UK;5. Department of Bacteriology, Nagoya University Graduate School of Medicine, 65 Tsurumai‐cho, Showa‐ku, Nagoya, Aichi 466‐8550, Japan |
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| Abstract: | Accurate and rapid detection of carbapenemases and identification of their types in Enterobacteriaceae are both still major challenges for clinical laboratories in attempting to prevent the intrusion and transmission of carbapenemase‐producing Enterobacteriaceae. This study aimed to evaluate the performance of the MASTDISCS combi Carba plus disc system in identification of different carbapenemase types, including OXA‐48‐type carbapenemase, for which no specific enzyme inhibitors have so far been available. The simple disc system discriminates carbapenemases, including OXA‐48‐types exhibiting low carbapenem minimum inhibitory concentrations, by targeting Enterobacteriaceae isolates with a EUCAST meropenem screening cut‐off of ≥0.25 mg/L. |
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| Keywords: | carbapenemase‐producing Enterobacteriaceae Klebsiella pneumoniae carbapenemase metallo‐β ‐lactamase OXA‐48 carbapenemase |
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