Prostaglandin E and mitogenic stimulation of human lymphocytes in serum-free medium |
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| Authors: | Tadao Okazaki Masathosi Shimizu Carl E. Arbesman Elliott Middleton |
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| Affiliation: | Department of Medicine, State University of New York at Buffalo, School of Medicine and the Allergy Research Laboratory at the Buffalo General Hospital, Buffalo, New York USA |
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| Abstract: | Sera used in cell cultures contain significant amount of prostaglandins (PGs). In order to vaoid any effects of contaminating PGs, the present study employed a serum-free culture medium and confirmed the inhibitory effect of prostaglandin E (PGE) on the human lymphocyte activation which had been observed previously employing a serum-containing medium. PGE1 displayed a significantly stronger inhibitory effect on the cells than previously shown. Furthermore, reported enhancement of PGE synthesis by mitogen-activated lymphocytes could not be reproduced. |
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