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Rapid isolation of kestose by low-pressure chromatography after enzymatic synthesis with invertase |
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| Authors: | Richard Idem Somiari Stanislaw Bielecki |
| Institution: | (1) Laboratory of Molecular Research, University of Maryland, Baltimore, 10 South Pine Street, MSTF 5-65, Baltimore, MD 21201, USA;(2) Institute of Technical Biochemistry, Technical University of Lodz, ul, Stefanowskiego 4/10, 90-924 Lodz, Poland |
| Abstract: | By using a two-step protocol involving flash chromatography on silica gel-60 and gel filtration on Biogel P2 we isolated 56 g pure kestose/L after enzymatic synthesis with 115 U invertase in media containing 50% (w/w) sucrose and 90% (v/v) butyl acetate. The isolated product is homogenous after high pH anion exchange chromatography with pulsed amperometric detection and is suitable for downstream utilization without further purification. |
| Keywords: | flash chromatography high pH anion exchange chromatography invertase Kestoses |
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