Changes in immunohistochemical distribution of cytochrome MC-P-448 in the rat liver during acclimation to cold |
| |
Authors: | Yasuhito Kobayashi Yutaka Motohashi Yoshifumi Miyazaki Kiyozo Kishi Takehito Takano |
| |
Affiliation: | (1) Department of Clinical Pathology, Saitama Cancer Center, 818, Komuro, Ina-cho, Kita-adachi-gun, 362 Saitama, Japan;(2) Department of Public Health and Environmental Science, School of Medicine, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, 113 Tokyo, Japan;(3) Laboratory of Biological Activities, Forestry and Forest Products Research Institute, 1, Matsunosato, Kukisaki-cho, Inashiki-gun, 305 Ibaraki, Japan |
| |
Abstract: | Immunohistochemical localization of cytochrome MC-P-448 (form of cytochrome P-450 induced by methylcholanthrene) in rat hepatic lobule and the changes in their distribution pattern in response to cold exposure at 4°C were investigated. The distribution of hepatocytes expressing immunoreactivity to cytochrome MC-P-448 was demonstrated with rabbit anti-MC-P-448 serum using a microphotomeasurement system P1 (Nikon). A duration of cold exposure for 1, 2, 3 or 4 weeks at 4°C was applied to study the effect of cold adaptation of cytochrome MC-P-448. In control rats housed at 24°C, hepatocytes showing high immunoreactivity to cytochrome MC-P-448 were located in the centrilobular areas of the hepatic lobules, whereas they disappeared markedly in the 4-week cold-exposed rats. In 1-week and 2-week cold-exposed rats, only a slight decrease in the expression of MC-P-448 positive hepatocytes was observed. These changes were clearly seen by visual inspection of the distribution topography as determined by a microphotomeasurement technique. In conclusion, cytochrome MC-P-448 forms which were predominantly located in centrilobular areas in the hepatic lobule decreased in 4-week cold-exposed rats. This was in contrast to our early report which showed an increasing tendency of cytochrome PB-P-450 forms in 4-week cold-exposed rats. |
| |
Keywords: | Cytochrome P-450 Cytochrome MC-P-448 Cold stress Liver immunohistochemistry |
本文献已被 SpringerLink 等数据库收录! |
|