1H, 15N, 13C assignment and secondary structure determination of two domains of La protein from D. discoideum |
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Authors: | Maria Apostolidi Dionysios J. Vourtsis Christos T. Chasapis Constantinos Stathopoulos Detlef Bentrop Georgios A. Spyroulias |
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Affiliation: | 1. Department of Biochemistry, School of Medicine, University of Patras, 26504, Patras, Greece 2. Department of Pharmacy, University of Patras, 26504, Patras, Greece 3. Institute of Physiology II, University of Freiburg, 79104, Freiburg, Germany
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Abstract: | Biosynthesis of RNA polymerase III transcripts requires binding of the La protein at their 3′ end. La is an abundant nuclear RNA-binding protein which protects the nascent transcripts from 3′ exonuclease degradation. Here, we report the high yield expression and preliminary structural analysis through NMR spectroscopy of two recombinant RNA binding domains (La motif and NRRM) from the La protein of Dictyostelium discoideum. Both recombinant protein constructs were well-folded and allowed for an almost complete sequence-specific assignment of the 15N and 13C labeled domains and their secondary structure prediction using PECAN online tool. |
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