Comparison between responses to gametophytic and sporophytic recurrent selection in maize (Zea mays L.) |
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| Authors: | P. Landi E. Frascaroli R. Tuberosa S. Conti |
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| Affiliation: | (1) Department of Agronomy and Plant Genetics and the Plant Molecular Genetics Institute, University of Minnesota, 55108 St. Paul, MN, USA;(2) Waksman Institute of Microbiology, Rutgers;(3) Present address: The State University of New Jersey, 08855 Piscataway, NJ, USA;(4) Present address: Plant Science Research, Inc., 10320 Bren Road, 55343 Minnetonka, MN, USA |
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| Abstract: | Summary Experiments were conducted to determine the chromosomal location of the gene conditioning overproduction of a methionine-rich, 10-K zein in maize kernels of line BSSS53. In addition, the chromosomal location of the structural gene encoding the overproduced protein was determined. Whereas the structural gene, designated Zps10/(22), was found to be located on the long arm of chromosome 9 near the centromere, the locus regulating overproduction of the zein protein was mapped to the short arm of chromosome 4. This regulatory gene has been designated Zpr10/(22). Regulation of 10-K zein production by Zpr10/(22) is, therefore, via a trans-acting mechanism. |
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| Keywords: | Zea mays L. Zein Protein isoelectric focusing (IEF) Restriction fragment length polymorphism (RFLP) mapping Trans-acting factors |
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