Production by non-O1 Vibrio cholerae of hemolysin related to thermostable direct hemolysin of Vibrio parahaemolyticus |
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Authors: | Myonsun Yoh Takeshi Honda Toshio Miwatani |
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Institution: | Department of Bacteriology and Serology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, 565 Japan |
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Abstract: | Abstract The production by non-O1 Vibrio cholerae of a hemolysin immunologically related to the thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus was demonstrated by enzyme-linked immunosorbent assay and a double gel diffusion test. Although results by the double gel diffusion test suggested the immunological identities of TDH and the TDH-related hemolysin of non-O1 V. cholerae , conventional polyacrylamide gel disc electrophoresis and immunoelectrophoresis suggested some differences between the two, at least with respect to charge. The TDH-related hemolysin of non-O1 V. cholerae was also shown to differ from the hemolysin of non-O1 V. cholerae reported previously. |
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Keywords: | Elisa NAG vibrio cytolysin |
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