Formation of deoxycytidine diphosphate-diglyceride by nuclei isolated from HeLa cells. |
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Authors: | M Yamada J Aucker A Weissbach |
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Affiliation: | Roche Institute of Molecular Biology, Nutley, New Jersey 07110 USA |
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Abstract: | Isolated nuclei from HeLa cells synthesize dCDP-diglyceride from dCTP at the rapid rate of 5–10 nmol/20 min/108 nuclei. The incorporation of dCTP into this phospholipid precursor is thus 10 to 20 times faster than the incorporation of dCTP into DNA, in vitro, under the same conditions. ATP, phosphatidic acid, and MgCl2 are required for optimal synthesis of dCDP-diglyceride. The reaction is completely inhibited by the presence of 0.04% Triton N-101. Liponucleotide formation occurs equally well with dCTP or CTP in this system and competition studies suggest that a single enzyme catalyzes the formation of dCDP- and CDP-diglyceride. |
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