表皮生长因子诱导人成纤维细胞基因表达谱的变化

深入研究EGF对靶细胞的作用有助于揭示细胞增殖、转化、凋亡以及胚胎发育的分子机理。本文采用cDNA微阵列技术,检测EGF持续作用48小时后人胚肺二倍体成纤维细胞基因表达谱的变化。结果显示:EGF长期作用诱导广泛的基因表达变化,在所检测的4096种人类基因中,有855种发生了变化,这些表达变化的基因参与细胞的能量代谢、生物合成、细胞周期调控及受体酪氨酸激酶和G蛋白耦连受体信号转导等细胞反应,其中最显著的变化趋势是GPCR及其相关蛋白基因表达的下调。

EFFECT OF EGF ON GENE EXPRESSION PROFILE OF HUMAN FIBROBLATS

A better understanding of the molecular affects of EGF on target cell can help to reveal important aspects of cellular proliferation, transformation and apoptosis, as well as embryonic and fetal development. In this study, we have examined differences in gene expression of cultured fibroblasts with EGF stimulation for 48 hours by using high-density cDNA microarrays . We found that EGF cause widespread alteration in gene expression. 855 genes, more than 40% of the assayed, showed changed expression, which are involved in various cellular process, such as, energetic metabolism, biosynthesis, the progress of cell cycle, and the signaling pathways of receptor tyrosine kinase (RTKs) and G protein- coupled receptors (GPCRs). The most striking finding is that long-term EGF treatment on cultured fibroblasts desensitize GPCRs to their physiological and nonphysiological stimuli by down-regulation of GPCRs and their associated proteins , which seems to be the slow-acting but permanent effect of EGF on GPCR signalling pathways.