Long-term preservation of freeze-dried mouse spermatozoa |
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Authors: | Kaneko Takehito Serikawa Tadao |
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Institution: | Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan. tkaneko@anim.med.kyoto-u.ac.jp |
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Abstract: | Many genetically engineered mice strains have been generated worldwide and sperm preservation is a valuable method for storing these strains as genetic resources. Freeze-drying is a useful sperm preservation method because it requires neither liquid nitrogen nor dry ice for preservation and transportation. We report here successful long-term preservation at 4 °C of mouse spermatozoa freeze-dried using a simple buffer solution (10mM Tris, 1mM EDTA, pH 8.0). Offspring with fertility were obtained from oocytes fertilized with freeze-dried spermatozoa from C57BL/6 and B6D2F1 mouse strains stored at 4 °C for 3 years. This freeze-drying method is a safe and economical tool for the biobanking of valuable mouse strains. |
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Keywords: | Mouse B6D2F1 C57BL/6 Sperm Freeze-drying Tris–EDTA buffer Long term preservation ICSI |
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