Continuous culture of suspended plant cells |
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Authors: | H. J. G. ten Hoopen W. M. van Gulik J. J. Heijnen |
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Affiliation: | (1) Biotechnology Delft Leiden, Project Plant Cell Biotechnology, Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands;(2) Kluyverlaboratorium voor Biotechnologie, Vakgroep Bioprocestechnologie, Julianalaan 67, 2628 BC Delft, The Netherlands |
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Abstract: | Summary Continuous culture is an attractive research tool in physiologic and growth and production kinetics research. However, fulfillment of the basic assumptions of continuous culture in the experimental set-up may cause problems. The homogeneity of plant cell cultures and effluent, particularly, may cause problems. This paper presents an experimental set-up which solves these problems and describes the use of this equipment in a study of the growth kinetics of plant cells. Industrial application of the continuous culture of plant cells in the production of secondary metabolites seems to be profitable when compared with batch or fed-batch cultures. However, various problems such as uncoupled product formation and strain instability make fed-batch culture a better choice. Presented in the Session-in-Depth Batch Production and Fermentation at the 1991 World Congress on Cell and Tissue Culture, Anaheim, California, June 16–20, 1991. |
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Keywords: | continuous culture plant cells growth kinetics secondary metabolites |
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