Development of a molecular marker associated with Verticillium wilt resistance in diploid interspecific potato hybrids |
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| Authors: | JinJoo Bae Dennis Halterman Shelley Jansky |
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| Institution: | (1) Department of Horticulture, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53706, USA;(2) Vegetable Crops Research Unit, U.S. Department of Agriculture-Agricultural Research Service, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53706, USA;(3) Department of Plant Pathology, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53706, USA |
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| Abstract: | Verticillium wilt (VW) is a widespread and serious potato (Solanum tuberosum) disease caused by the soilborne fungi Verticillium dahliae and V. albo-atrum. Breeding for VW resistance in potato is challenging due to ambiguous symptom expression, a lack of high throughput screening
techniques, and variability in colonization by the fungus among and within plants. Genetic studies have identified major genes
that confer resistance in diploid Solanum chacoense (V
c
) and interspecific hybrids (V
w
and V
t
). However, to date, these genes have not been used to develop molecular markers for the identification of resistant clones.
Tomato Ve1 and Ve2 gene sequence information was used to amplify candidate Ve gene orthologs from both resistant and susceptible diploid potato hybrids. A CAPS marker was generated to track VW resistance
in a backcross population segregating for resistance. The marker was also tested for its usefulness in other breeding lines.
Our results indicate that this marker is effective for selection of the V
w
gene in segregating breeding populations. |
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| Keywords: | Verticillium wilt Interspecific potato hybrids Disease resistance CAPS marker Dosage effect Ve resistance gene |
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