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转抗虫基因欧美黑杨离体快繁技术研究
引用本文:康冰 曹建军 等. 转抗虫基因欧美黑杨离体快繁技术研究[J]. 西北植物学报, 2002, 22(6): 1474-1478
作者姓名:康冰 曹建军 等
作者单位:西北农林科技大学生命科学学院,陕西杨陵,712100
摘    要:以抗虫欧美黑杨的叶,带腋芽茎段为外植体进行离体快繁技术研究。最佳接种时间为8月份,新芽生长迅速。基本培养基为MS,较适初培养基为MS+6-BA0.5mg/L(以下单位同)+NAA0.01mg/,附加30g/L,蔗糖,7g/L琼脂。愈伤组织诱导并同时分化出新芽培养基为MS+6-BA1.5 NAA0.3,附加40g/L蔗糖,6g/L琼脂。继代增殖培养基为MS 6-BA1.0 NAA0.1 GA2.0,附加30g/L蔗糖,5g/L琼脂。生根培养基为MS+IBA2.0。

关 键 词:转抗虫基因欧美黑杨 离体快繁技术 新芽增殖 生根培养
文章编号:1000-4025(2002)06-1474-05
修稿时间:2002-06-03

In vitro micropropagation of insect-resistant transgenic Populus euramericana
KANG Bing,CAO Jian jun,ZHANG Xiao hong,LI Jun chao,QI Lan lan,SUN Jian zhao,GUI Lan fang,DONG Xiu hua. In vitro micropropagation of insect-resistant transgenic Populus euramericana[J]. Acta Botanica Boreali-Occidentalia Sinica, 2002, 22(6): 1474-1478
Authors:KANG Bing  CAO Jian jun  ZHANG Xiao hong  LI Jun chao  QI Lan lan  SUN Jian zhao  GUI Lan fang  DONG Xiu hua
Abstract:Shoots and axillary buds were induced in vitro from apexes,stem segments and leaves of insect-resistant transgenic Populus euramericana .August is the best inoculated time and new buds grew rapidly.Basic medium is MS medium.The proper initial culture medium is MS+6 BA 0.5 mg/L+NAA 0.01 mg/L,buds were regenerated from the explants.Calluses were induced and meanwhile cluster adventitious buds were formed on the MS basic containing 1.5 mg/L 6 BA and 0.3 mg/L NAA,high sugar concentration (40 mg/L) is suitable for calluses inducing and new buds forming.The medium for multiplication is MS+6 BA 1.0 mg/L+NAA 0.1 mg/L+GA 2.0 mg/L.The rooting medium is MS+IBA 2.0 mg/L.
Keywords:Populuas euramericana  insect-resistant transgenic  tissue culture  regeneration  rooting
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