培育无选择标记的转基因植物

目前,几乎所有的植物遗传转化都要通过使用选择标记基因,如抗生素或除草剂抗性基因等来筛选转化子,虽然没有研究结果表明选择标记基因影响人类健康或环境安全,但近年来也引发了人们对转基因产品安全性的担心。为了消除公众对转基因食品的安全性顾虑,无选择标记的转基因植物应运而生。本文综述了共转化系统、位点特异性重组系统(包括FLP/FRT、Cre/lox、R/RS及Gin/gix系统)和转座子系统(Ac/Ds转座子系统)在培育无选择标记转基因植物中的应用。

The Cultivation of Marker-Free Transgenic Plants

In present,all the transgenic plants are almost using the selectable marker genes such as antibiotic and herbicide resistance to select transplants.Recent years,more and more people worry about the safety of transgenic product,although there is no researches have showed that selectable marker genes can affect the human health and the environment security.Marker-free is a rapidly developed method which can eliminate the safety concern of transgenic food.The using of co-transformation system,site-specific recombination system(FLP/FRT?Cre/lox?R/RS and Gin/gix systems)and transposable element system(Ac/Ds transposable element system)in breeding marker-free transgenic plants were reviewed in this paper.