Effect of formulation on the viability of Metarhizium anisopliae conidia |
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Authors: | R A Daoust M G Ward D W Roberts |
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Institution: | Fukushima-ken Sericultural Experiment Station, Yanagawa-machi, Date-gun, Fukushima 960-07, Japan;Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan |
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Abstract: | A slide agglutination test using antibody-sensitized latex particles was developed for the specific detection in the early infection of the flacherie virus of the silkworm, Bombyx mori. With this test, 0.63 μg/ml of virus protein could be detected. The tests was completed within 5 min. Extracts from flacherie virus-infected silkworm larvae agglutinated latex particles specifically, while there was no agglutination by extracts of normal and nuclear polyhedrosis virus-infected silkworm larvae. The results showed that the sensitivity and simplicity of this technique for the detection of flacherie virus were greater than those of conventional serological techniques such as the immunofluorescence test and the immunodiffusion test. |
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Keywords: | Latex agglutination virus detection silkworm flacherie virus |
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