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Partial trisomies in two spontaneously arising long-lived human keratinocyte lines
Authors:J Donald Weaver  G Stetten  J W Littlefield
Institution:(1) Departments of Physiology, Johns Hopkins University School of Medicine, 21205 Baltimore, Maryland;(2) Gynecology and Obstetrics, Johns Hopkins University School of Medicine, 21205 Baltimore, Maryland
Abstract:Summary During experiments concerning the introduction of oncogenes into normal human keratinocytes, we observed long-lived colonies arising spontaneously at the same low frequency in control cultures as in those transfected with Ha-rasEJ or activated c-myc or both. Two of these were karyotyped early in their life span and showed additional chromosomal material on the short arm of chromosome 9 in one case and of chromosome 18 in the other, whereas the parental cells had a normal karyotype. This indicates the presence of a partial trisomy in each line, although the origin of the extra chromosomal material is not known. A similarly long-lived human keratinocyte line containing an isochromosome of the long arm of chromosome 8 has been described elsewhere. Together these results suggest that the spontaneous occurrence of long-lived lines is more common in human keratinocytes than in fibroblasts and that a triple dose of one or more genes may be the initial event in this process. This work was supported by grant CA16754 from the National Cancer Institute, Bethesda, MD.
Keywords:keratinocytes  long-lived  partial trisomy  senescence  immortalization  transformation
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