Purification of eukaryotic ribosomes by isopycnic centrifugation in sucrose |
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Authors: | P Nieuwenhuysen H Slegers |
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Institution: | Department of Cell Biology, University of Antwerp, B-2610 Wilrijk, Belgium |
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Abstract: | A simple and inexpensive procedure for the isolation and purification of ribosomes from eukaryotes is described. The method avoids pelleting of ribosomes at high centrifugal forces and involves isopyenic centrifugation of the post-mitochrondrial supernatant in sucrose, precipitation of ribosomes with 10% polyethylene glycol, and zonal sucrose gradient centrifugation. The ribosomes obtained in this way are very pure and thus especially suited for the measurements of physical properties. The isopycnic centrifugation can also be used for the purification of other macromolecules and is only limited by a maximum density of sucrose of 1.40 g/cm3 obtained at the bottom of the centrifugation tubes. |
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Keywords: | Author to whom all correspondence should be addressed |
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