Quantitative determination of prostaglandins E1 and F1α by multiple ion analysis |
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Authors: | Marc E Goldyne Sven Hammarström |
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Institution: | Department of Chemistry, Karolinska Institutet, S-104 01 Stockholm, Sweden |
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Abstract: | Quantitative assays for prostaglandins (PG) E1 and PGF1α are described using 3,3,4,4,5,6-2H6]labeled prostaglandins as carriers and methyl ester-O-methyloxime-acetate (PGE1) and methyl ester-acetate (PGF1α) derivatives for gas - liquid chromatography/mass spectrometric analysis. Thin-layer argentation chromatography was used to separate PGE1 from PGE2 and 13, 14-dihydro-PGE2. These latter compounds, which do not separate from PGE1 using conventional thin-layer chromatography or under the gas - liquid chromatographic conditions used, can significantly interfere with the quantitative analysis of PGE1. The method described prevents this interference and is therefore suitable for the accurate analysis of PGE1 in biological samples containing a high concentration of PGE2 and/or 13, 14-dihydro-PGE2. |
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