The isolation of microsomal subfractions of mouse plasmacytoma cells: The effect of salt concentration during nitrogen cavitation |
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Authors: | Asbjörn M Svardal Ian F Pryme |
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Institution: | Cellebiologisk Forskningsgruppe, Biokiemisk Institutt, De Prekliniske Institutter, Universitetet i Bergen, 5000 Bergen, Norway |
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Abstract: | Following disruption of MPC-11 cells by nitrogen cavitation the microsomes have been fractionated by centrifugation on discontinuous sucrose gradients. When the homogenization buffer contained 25 mm KCl three fractions were observed: smooth microsomes, light rough microsomes, and heavy rough microsomes. When it contained 100 mm KCl, however, only smooth and light microsomal fractions were found. Under the latter conditions the heavy rough microsomal vesicles were apparently not released as separate organelles but instead sedimented together with the endoplasmic reticulum which remains attached to the nuclei. |
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Keywords: | Address for correspondence: Ian F Pryme Department of Biochemistry University of Bergen Årstadveien 19 5000 Bergen Norway |
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