A new fluorometric method for determination of picomoles of inorganic phosphorus. Application to the renal tubular fluid |
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| Authors: | Michele G. Brunette Normand Vigneault Gaby Danan |
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| Affiliation: | Department of Rediatrics, Maisonneuve Hospital, University of Montreal, School of Medicine, Montreal, Canada |
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| Abstract: | A new method for the determination of inorganic phosphorus (Pi) in 4 nl of renal tubular fluid is described. Phosphate is converted to hexadimolybdatophosphate, which is reacted with thiamine to produce a highly fluorescent thiochrome. The reaction is stable and reproducible. Problems of interference are minimal. Microdeterminations of Pi in the proximal tubules and glomerular filtrates of the rat yielded results similar to those published in the literature. |
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