Analysis of radioactive and nonradioactive purine bases,nucleosides, and nucleotides by high-speed chromatography on a single column |
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| Authors: | Bohdan Bakay Erkki Nissinen Lawrence Sweetman |
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| Institution: | Department of Pediatrics, University of California at San Diego, La Jolla, California 92093 USA |
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| Abstract: | The concentrations of radioactive and nonradioactive purine bases, purine nucleosides, purine mono-, di-, and trinucleotides in acid extracts of fibroblasts were determined by anion-exchange column chromatography. The concentrations of nonradioactive components were determined by computerized integration of the signal from a double-beam uv-detector. The radioactive metabolites were quantitated by high-efficiency, continuous liquid scintillation counting, employing a discrete sample transport system. |
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| Keywords: | Please direct reprint requests to this author |
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