A simple method for synthesizing [γ-32P]nucleoside triphosphates using [32P]acetylphosphate and acetate kinase |
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| Authors: | Pál I Bauer Gyula Várady |
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| Institution: | Second Institute of Biochemistry, Semmelweis University, Medical School, 1088 Budapest, Hungary |
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| Abstract: | A simple, rapid, and inexpensive method is described for the synthesis of γ-32P-labeled ribo- or deoxyribonucleoside triphosphates. The procedure involves chemical synthesis of 32P]acetylphosphate and subsequent phosphorylation of nucleoside diphosphates using acetate kinase (EC 2.7.2.1) and a final purification step. The entire procedure is performed 8 h or less. |
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