Analysis of nucleic acid bases by high-performance liquid ion chromatography |
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| Authors: | V.P. Demushkin Yu.G. Plyashkevich |
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| Affiliation: | Shemyakin Institute of Bioorganic Chemistry of the USSR Academy of Sciences, Vavilova 32, 117312 Moscow, USSR |
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| Abstract: | Optimal conditions for the high-speed chromatography of nucleic acid bases using cation exchangers are described. Bases are resolved on Aminex A-7 with 1 m phosphate in 20% ethanol at pH 3.4 and 70°C and with 1 m phosphate at pH 2.75 and 50°C. The optimal conditions for the same analysis on the anion exchanger Aminex A-28 are by elution with 0.4 m phosphate-borate, pH 8.25, at 45°C. The dependence of the charge of various analogs of the nucleic acid bases on the pH of the solution is tabulated. |
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