A borate column for the separation of ribo and deoxyribonucleosides |
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| Authors: | Richard G Moran William C Werkheiser |
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| Institution: | 1. Department of Experimental Therapeutics, Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York 14263 USA;2. Grace Cancer Drug Center, Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York 14263 USA |
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| Abstract: | A method is described for the separation of mixtures of the naturally occurring ribo- and deoxyribonucleosides by chromatography on columns of Dowex 50 eluted with ammonium borate. Nucleotides, ribonucleosides, and nucleotide-containing complex sugars are not retained on the column under these conditions, permitting unambiguous separation of these compounds from the deoxyribonucleosides, which are easily separated from each other by this chromatography. The elution positions of the nucleic acid bases allow partial to complete resolution from the corresponding deoxynucleosides. This column has either preparative or analytical utility for the procurement of nucleoside fractions free of the common contaminants. |
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