The enzymic synthesis of β-[32P]UDP-N-acetylglucosamine |
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| Authors: | John Heptinstall Philip J. Ward Ian C. Hancock |
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| Affiliation: | Microbiological Chemistry Research Laboratory, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, United Kingdom |
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| Abstract: | Cells of Micrococcus sp. 2102 incorporate inorganic [32P]phosphate from the medium into the sugar-phosphate polymer of the wall. Controlled acid hydrolysis of sodium dodecyl sulphate-extracted cells gives N-acetylglucosamine 6-[32P]phosphate which can be purified by ion-exchange chromatography and incubated with UTP in the presence of crude preparations of phosphoacetylglucosamine mutase from Neurospora crassa and UTP: N-acetylglucosamine 1-phosphate phosphotransferase from Bacillus licheniformis which act in concert to synthesise β-[32P]UDP-N-acetylglucosamine. |
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