The enzymic synthesis of β-[32P]UDP-N-acetylglucosamine |
| |
| Authors: | John Heptinstall Philip J Ward Ian C Hancock |
| |
| Institution: | Microbiological Chemistry Research Laboratory, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, United Kingdom |
| |
| Abstract: | Cells of Micrococcus sp. 2102 incorporate inorganic 32P]phosphate from the medium into the sugar-phosphate polymer of the wall. Controlled acid hydrolysis of sodium dodecyl sulphate-extracted cells gives N-acetylglucosamine 6-32P]phosphate which can be purified by ion-exchange chromatography and incubated with UTP in the presence of crude preparations of phosphoacetylglucosamine mutase from Neurospora crassa and UTP: N-acetylglucosamine 1-phosphate phosphotransferase from Bacillus licheniformis which act in concert to synthesise β-32P]UDP-N-acetylglucosamine. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
