Neural precursors express multiple chondroitin sulfate proteoglycans, including the lectican family |
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Authors: | Kabos Peter Matundan Harry Zandian Mandana Bertolotto Cristina Robinson Michael L Davy Brian E Yu John S Krueger Richard C |
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Institution: | Department of Pediatrics, Division of Molecular and Human Genetics, Children's Research Institute, The Ohio State University, Columbus, OH, USA. |
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Abstract: | Chondroitin sulfate proteoglycans (CSPGs) abnormally accumulate in cerebrospinal fluid (CSF) of both human neonates with preterm hydrocephalus, and P8 hydrocephalic mice. We hypothesized CSF CSPGs are synthesized by neural precursors, separated from ventricular CSF by ependyma, which is often disrupted in hydrocephalus. Western blotting demonstrates that neural precursors cultured as neurospheres secrete CSPGs (> 30 microg/ml) into their media which appear to be very similar to these CSF CSPGs. Some CSPGs bear the stage-specific embryonic antigen-1 (ssea-1), associated with embryonic/neural stem cells. Neurospheres transcribe many CSPG genes, including the entire aggrecan/lectican family, phosphacan, and tenascin. Phosphacan can be detected in media by Western blotting. Aggrecan can be detected in media after purification using hyaluronic acid affinity chromatography. During differentiation, neurospheres downregulate CSPGs. This is the first report to show that proliferating neural precursors synthesize lecticans, including aggrecan, which are downregulated with differentiation. These observations suggest novel links between CSPGs and CNS precursor biology. |
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Keywords: | Aggrecan Lectican Phosphacan Cerebrospinal fluid Hydrocephalus LeX/ssea-1 Neurospheres |
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