Giant cells fromSaccharomyces uvarum grown after X-irradiation |
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Authors: | PD Dr Hermann Rink Dipl-Biol H -J Partke |
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Institution: | 1. Institut für Biophysik, Abteilung für Strahlenbiologie, Universität Bonn, D-5300, Bonn Annaberger Weg 15, Germany
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Abstract: | Yeast cells, Saccharomyces uvarum, were irradiated with X-rays and grown in liquid suspension. Glucose as the only carbon source was limited to 12.5 mM. Under these conditions giant cells are formed. Cell number, glucose utilization, ethanol production and oxygen consumption are measured during the time of growth. The mean weight of single cells in the stationary phase increases up to 75 krad and is not due to an uptake of water. In irradiated cultures oxygen consumption and glucose utilization per cell are higher than in control cells. The data demonstrate that synthesis- and energy-metabolism during the formation of non-dividing, radiation-induced giant cells is increased. |
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