火棘花挥发油化学成分的GC-MS分析及抗氧化活性研究 |
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引用本文: | 葛丽娜,韩雪,任珂珂,章甫,彭永宇,毕淑峰. 火棘花挥发油化学成分的GC-MS分析及抗氧化活性研究[J]. 植物研究, 2014, 34(2): 276-281 |
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作者姓名: | 葛丽娜 韩雪 任珂珂 章甫 彭永宇 毕淑峰 |
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作者单位: | 黄山学院生命与环境科学学院,黄山 245041 |
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基金项目: | 中国博士后科学基金项目(2011M500898);国家级大学生创新计划项目(201210375041) |
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摘 要: | 采用水蒸气蒸馏法提取火棘花挥发油,利用气相色谱—质谱联用技术(GC-MS)分析其化学成分。使用维生素C和BHT为阳性对照,以DPPH自由基、亚硝酸钠清除作用为指标评价挥发油的抗氧化活性。从挥发油中鉴定了77个化合物,占挥发油总量的83.77%,含有多种生物活性成分,以萜类及其含氧衍生物(50.31%)、烷烃(18.52%)、醛(5.54%)为主;挥发油对DPPH自由基、亚硝酸钠有明显的清除作用,清除率为50%时,其体积分别为43.51、79.48μL,样品量与清除率间呈量效关系;挥发油对DPPH自由基的清除效果略低于1 mg·mL-1维生素C,对亚硝酸钠的清除效果优于1 mg·mL-1的BHT。
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关 键 词: | 火棘花 挥发油 化学成分 GC-MS 抗氧化活性 |
GC-MS Analysis on Chemical Constituents and Antioxidant Activity of Volatile Oil from Pyracantha fortuneana Flowers |
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Affiliation: | College of Life and Environment Science,Huangshan University,Huangshan 245041 |
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Abstract: | The volatile oil from Pyracantha fortuneana flowers was extracted by water steam distillation and analyzed by GC-MS. The antioxidant activity of volatile oil was evaluated by DPPH·and sodium nitrite scavenging capacity with VC and BHT as the positive controls. Seventy-seven compounds, which occupied 83.77% of total constituents and contained many bioactive constituents, were identified. Terpenes and their derivatives (50.31%), alkanes (18.52%), and aldehydes (5.54%) were found to be dominant components in the volatile oil. The volatile oil exhibited significant scavenging capacity against DPPH·and sodium nitrite in a concentration-dependent fashion. When the scavenging rate against DPPH· and sodium nitrite of volatile oil was 50%, the volumes of volatile oil were 43.51 and 79.48 μL, respectively. The scavenging capacity against DPPH· of volatile oil was slightly worse than that of 1 mg·mL-1 Vc and the scavenging capacity against sodium nitrite of volatile oil was better than that of 1 mg·mL-1 BHT. |
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Keywords: | Pyracantha fortuneana flowers volatile oil chemical constituents GC-MS antioxidant activity |
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