Crystallization and Preliminary X-Ray Diffraction Analysis of Group 5 Mite Allergen fromDermatophagoides pteronyssinus |
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| Authors: | Shwu-Huey Liaw Hsin-Zu Chen I-Chun Kuo Kaw-Yan Chua |
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| Institution: | aInstitute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan;bInstitute of Immunology, College of Medicine, National Taiwan University, Taipei, Taiwan |
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| Abstract: | Crystals of the 14-kDa group 5 allergen fromDermatophagoides pteronyssinus(Der p 5) have been obtained at low pH and diffract to 3-Å resolution using a conventional x-ray source. The crystals belong to tetragonal space group P41212 or P43212, with unit cell parametersa=b= 114 Å andc= 234 Å. A self-rotation search revealed a 432 point symmetry and thus suggested 96 molecules in one unit cell, hence 12 monomers in each asymmetric unit. |
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| Keywords: | Der p 5 mite allergen x-ray crystallography |
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