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一种快速检测分离溶藻细菌方法的初探
引用本文:吕伟英,赵以军,周瑞,吴广涛,胡蝶,吴刚. 一种快速检测分离溶藻细菌方法的初探[J]. 微生物学通报, 2007, 34(1): 0119-0122
作者姓名:吕伟英  赵以军  周瑞  吴广涛  胡蝶  吴刚
作者单位:1. 华中师范大学生命科学学院,武汉,430079
2. 华中农业大学生命科学技术学院,武汉,430070
基金项目:国家自然科学基金;湖北省武汉市科技攻关项目
摘    要:传统的细菌培养基对铜绿微囊藻具有毒性作用。会大大影响溶藻细菌的筛选效率和准确性。通过基本培养基各成分对铜绿微囊藻DS的作用研究发现,培养基中的葡萄糖成分对藻有抑制作用,并且这种抑制作用与培养基中的葡萄糖浓度密切相关,当培养基中葡萄糖的浓度在0.1~0.4g/L时,藻细胞生长受到抑制,当用柠檬酸三钠取代葡萄糖后,铜绿微囊藻在改良后的培养基中生长正常,与对照组相比无显著性差异(P<0.05)。用改良的培养基富集水样中的溶藻微生物,并用此培养液直接感染宿主藻,一周内即可初步快速检测是否含有溶藻细菌。此种方法既排除了培养基的干扰因素,又迅速增加了溶藻细菌的生物量,并可大量收集细菌分泌的胞外物质,为溶藻细菌尤其以分泌物质溶藻的细菌的初步筛选提供了一条快捷、有效的途径。

关 键 词:溶藻细菌  微囊藻  葡萄糖  快速检测
文章编号:0253-2654(2007)01-0119-04
修稿时间:2006-03-08

A Methodological Study on Rapid Identification and Isolation of Algicidal Bacteria
LV Wei-Ying,ZHAO Yi-Jun,ZHOU Rui,WU Guang-Tao,HU Die and WU Gang. A Methodological Study on Rapid Identification and Isolation of Algicidal Bacteria[J]. Microbiology China, 2007, 34(1): 0119-0122
Authors:LV Wei-Ying  ZHAO Yi-Jun  ZHOU Rui  WU Guang-Tao  HU Die  WU Gang
Affiliation:College of Life science.Huazhong Normal University.Wuhan 430079;College of Life science.Huazhong Normal University.Wuhan 430079;College of Life science.Huazhong Normal University.Wuhan 430079;College of Life Science and Technology.Huazhong Agricultural University; Wuhan 430070;College of Life science.Huazhong Normal University.Wuhan 430079;College of Life science.Huazhong Normal University.Wuhan 430079
Abstract:In order to increase the efficiency of algicidal bacteria isolation , The components of minimal medium for isolation of algicidal bacteria were tested for the effection growth of Microcystis aeruginosa DS and a modified minimal medium was established.. The results indicated that glucase at the concentration of 0. 1-0. 4g/L in minimal medium inhibited the growth of Microcystis aeruginosa DS , and a modified minimal medium was developed using sodium citrate instead of glucoso as carbon sourso. On modified medium algicidal bacteria could be successfully isolated directly from field sample in 1 week. Microcystis aeruginosa DS could grow well on modified medium and the algicidal materials were secreted into medium by bacteria and the growth medium could be directly used for algicidal test.
Keywords:Algicidal bacteria  Microcystis  Glucose  Rapid identification
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