Two-step purification of low-content cellular protein using protein-imprinted polymers |
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| Authors: | Long Yi Xing Xiaocui Han Ruifang Sun Yang Wang Ying Zhao Zhuo Mi Huaifeng |
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| Institution: | aBiochemical Section of Key Laboratory of Functional Polymer Materials, the Ministry of Education of China, Institute of Polymer Chemistry, Nankai University, 300071 Tianjin, People’s Republic of China |
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| Abstract: | We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPs) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namely assistant recognition polymer chains, and uses cloned bacterial protein as a template. The low-content cellular target protein was purified from cell extract by this method. This is believed to be the first time that low-content cellular protein has been purified by using PIPs and with only two steps. |
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| Keywords: | Protein-imprinted polymer Assistant recognition polymer chains (ARPCs) Purification of cellular protein Cloned bacterial protein as template |
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