Roles of Peripheral P2X and P2Y Receptors in the Development of Melittin-Induced Nociception and Hypersensitivity |
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Authors: | Zhuo-Min Lu Fang Xie Han Fu Ming-Gang Liu Fa-Le Cao Jian Hao Jun Chen |
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Institution: | (1) Institute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders, Tangdu Hospital, Fourth Military Medical University, #1 Xinsi Road, Baqiao, Xi’an, 710038, People’s Republic of China;(2) Institute for Biomedical Sciences of Pain, Capital Medical University, Beijing, 100069, People’s Republic of China |
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Abstract: | A recent report from our laboratory shows that subcutaneous (s.c.) injection of melittin could induce persistent spontaneous
nociception (PSN) and primary thermal or mechanical hyperalgesia. However, the exact peripheral mechanisms underlying melittin-induced
multiple pain-related behaviors remain unclear. In this study, behavioral tests combined with pharmacological manipulations
were used to explore potential roles of local P2X and P2Y receptors in melittin-induced inflammatory pain and hyperalgesia.
Post-treatment of the primary injury site with s.c. injection of A-317491 (a potent P2X3/P2X2/3 receptor antagonist) and Reactive Blue 2 (a potent P2Y receptor antagonist) could significantly suppress the development
of melittin-evoked PSN and hypersensitivity (thermal and mechanical). Our control experiments demonstrated that local administration
of either antagonist into the contralateral hindpaw produced no significant effect on any kind of pain-associated behaviors.
Taken together, these data indicate that activation of P2X and P2Y receptors might be essential to the maintenance of melittin-induced
primary thermal and mechanical hyperalgesia as well as on-going pain.
Z.-M. Lu and F. Xie are contributed equally to the work.
Special issue article in honor of Dr. Ji-Sheng Han. |
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Keywords: | Bee venom Melittin Adenosine triphosphate (ATP) Persistent spontaneous nociception Primary thermal hyperalgesia Primary mechanical hyperalgesia |
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