| 江浙蝮蛇蛇毒中抗肿瘤蛋白的分离纯化及活性研究 |
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| 引用本文： | 王春玲,曹小红,闫仲丽.江浙蝮蛇蛇毒中抗肿瘤蛋白的分离纯化及活性研究[J].中国生物工程杂志,2005,25(B04):278-281. |
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| 作者姓名： | 王春玲 曹小红 闫仲丽 |
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| 作者单位： | 天津科技大学食品科学与生物技术学院,天津300222 |
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| 摘 要： | 利用离子交换和凝胶过滤层析等分离技术从江浙蝮蛇蛇毒中分离纯化到一种抗肿瘤蛋白，经SDS-聚丙酰胺凝胶电泳检测其分子量为58．2ku。MTT法测定该蛋白对K562细胞的LC50为4．96μg／mL，电子显微镜观察其能引起K562细胞的凋亡，琼脂糖凝胶电泳可见典型的DNA梯状条带，实验结果表明该蛋白对K562细胞有明显的抑制作用。
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| 关 键 词： | 江浙蝮蛇（Agkistrodom halys Pallas） 分离纯化 抗肿瘤 |
| 收稿时间： | 2004-10-29 |
| 修稿时间： | 2004-12-07 |
Study on Purification and Anti-tumor Activity of Agkistrodom halys Pallas |
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| WANG Chun-ling ,CAO Xiao-hong ,YAN Zhong-li.Study on Purification and Anti-tumor Activity of Agkistrodom halys Pallas[J].China Biotechnology,2005,25(B04):278-281. |
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| Authors: | WANG Chun-ling CAO Xiao-hong YAN Zhong-li |
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| Institution: | Food Science and Bioengineering Department of Tianjin University of Science and Technology, Tianjin 300222, China |
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| Abstract: | A protein was purified from Agkistrodom halys Pallas, which can inhibited K562 cells proliferation. Agkistrodom halys Pallas venom was firstly isolated by ultrofiltration following by ion exchange chromatography of DEAE-Sepharose, after chromatography of SephadexG-75 and reverse phase HPLC of C18 column, the protein was obtained, by means of SDS-PAGE, the relative molecular mass is 58.2ku. After treated by the protein, the cellular changes of K562 cell were observed with electric microscope, the apoptotic effect on k562 cell was observed by agarose gel electrophoresis of DNA fragments, and the LC50 was 4.96ug/mL, the results showed that the protein purified from has anti-tumor effect on K562 cell. |
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| Keywords: | Agkistrodom halys Pallas Purification Anti-tumor |
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