激素等外源物质对马铃薯愈伤组织花色苷积累的影响

从来源于马铃薯(Solanum tuberosum cv.Chieftain)茎的愈伤组织中分离到白色和红色2种愈伤组织,用鲜重法和分光光度法分别测量愈伤组织的生长量和花色苷的含量,并对激素、抗菌素和糖对马铃薯愈伤组织生长和花色苷积累的影响进行研究。结果表明:低浓度的2,4-D有利于红色愈伤组织的花色苷积累,高浓度的2,4-D促进其生长而不利于花色苷的积累;高浓度的6-BA能促进红色愈伤组织中花色苷的积累并诱导白色愈伤组织花色苷的合成,但抑制其生长;卡那霉素能使白色愈伤组织变红并积累花色苷,高浓度的卡那霉素严重抑制愈伤组织的生长并最终变褐死亡;提高蔗糖浓度能促进愈伤组织花色苷的产生和积累,但超过70g/L时抑制生长。实验结果为今后花色苷生物合成机理研究和花色苷的工厂化生产奠定了基础。

Effect of Exotic Matter on Anthocyanin Production in Potato Tissue Culture

The white and red calli were isolated from the callus of the stem explants of potatoes (Solanum tuberosum cv.Chieftain).The effects of growth regulators,antibiotic and sucrose on callus growth and anthocyanin content in callus cultures were studied by measurement of fresh weight and spectrophotometric assays.The results indicated that the lower contents of 2,4-D promote accumulation of anthocyanins in red callus.The higher contents of 2,4-D stimulate callus growth and inhibit anthocyanin production in red callus.The higher contents of 6-BA promote accumulation of anthocyanins in red callus,induce anthocyanin production in white callus and inhibit callus growth.The white callus can be induced to turn red and accumulate anthocyanins by different concentration of kanamycin.The higher contents of kanamycin inhibit callus growth and turn brown to lead callus dead eventually.The elevated contents of sucrose can stimulate anthocyanin production,while more than 70 g/L inhibit callus growth.The results of this study have laid the foundation for the investigation of the control mechanism of anthocyanin biosynthesis and colorant production in a factory by setting an efficient tissue culture system in the future.