Efficient expression of the yeast metallothionein gene in Escherichia coli. |
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Authors: | T Berka A Shatzman J Zimmerman J Strickler and M Rosenberg |
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Institution: | Department of Molecular Genetics, Smith Kline & French Laboratories, Swedeland, Pennsylvania 19406. |
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Abstract: | The yeast metallothionein gene CUP1 was cloned into a bacterial expression system to achieve efficient, controlled expression of the stable, unprocessed protein product. The Escherichia coli-synthesized yeast metallothionein bound copper, cadmium, and zinc, indicating that the protein was functional. Furthermore, E. coli cells expressing CUP1 acquired a new, inducible ability to selectively sequester heavy metal ions from the growth medium. |
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