C_(60)对小鼠S_(180)肉瘤光动力学作用模型的建立

为验证Ｃ６０对活体肿瘤的光动力学损伤作用，我们从两方面进行实验：Ｃ６０对荷瘤小鼠的Ｓ１８０实体瘤的光动力学杀伤作用和Ｃ８０对离体Ｓ１８０肿瘤细胞的光动力学杀伤作用，在小鼠的瘤体上注射Ｃ８０光敏剂，在５１１ｎｍ、５７８ｎｍ混合黄录色激光照射正派主发Ｃ６０，产生大量的单线态氧，杀伤活性肿瘤。激光光强为５００ｍＷ，Ｃ６０浓度为３０μｇ／ｍｌ时，荷瘤小鼠寿命平均延长５天，瘤径减小１ｃｍ，瘤重减轻０．８克，

MODEL OF C_(60) PHOTODYNAMIC EFFECT ON S_(180) TUMOR OF MOUSE

To prove C60 photodynamic effect on tumor cell, i.e. the photodynamic effect on mouse S180 tumor in vivo and the photodynamic effect on S180 tumor cells in vitro we injected the C60-liposome into the mouse S180 tumor body. We found that with green laser(511nm & 578nm)illumination the mouse S180 tumor body was strongly damaged. Under the intensity of 500mW and the concentration of C60(30ug/ml),mouse average survival time extended 5 days and the turmor size reduced 1cm and tumor weight decreased 0.8g. In the other way S180 tumor cells was co-cultured in vitro with C60-liposome for two days. The result of the killing was examed by MTT method and FDA-PI double staining method after illuminating the C60 with I-W lamp. S180 tumor cell in vitro were obviously damaged. C60 is a kind of strong hotosensitizer. It can kill mouse S180 tumor cells both in vivo and in vitro.