Analyses in transgenic tobacco of the promoter from a Brassica napus gene highly expressed in the stigma |
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| Authors: | L S Robert M Lévesque-Lemay J L Gerster H-P Hong W Keller |
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| Institution: | (1) Eastern Cereal and Oilseed Research Centre, K. W. Neatby Bldg, 960 Carling Ave, Ottawa, Ontario, K1A 0C6, Canada Fax: (613) 759-1701 e-mail: robertls@em.agr.ca, CA;(2) Plant Biotechnology Institute, NRC, 110 Gymnasium Place, Saskatoon, Sask., Canada S7N 0W9, CA |
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| Abstract: | A genomic clone, Pis G363, containing the Brassica napus stigma-expressed gene Pis 63-2 was isolated and sequenced. The coding region of Pis G363 does not possess introns and shows
82% identity to the nucleotide sequence of a gene from Arabidopsis BAC clone T01B08. A 2-kb promoter fragment from Pis G363 was fused to the coding sequence of the marker enzyme β-glucuronidase (GUS) and introduced into tobacco via Agrobacterium-mediated transformation. The promoter fragment directed expression of the GUS gene in the stigma of transgenic tobacco. Some
transformants also showed relatively low GUS activity in the pollen.
Received: 25 May 1998 / Revision received: 30 July 1998 / Accepted: 21 August 1998 |
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| Keywords: | Brassica β -glucuronidase Promoter Stigma Tobacco |
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