百合鳞片离体培养诱导小鳞茎发生的研究

百合鳞片离体培养诱导愈伤组织和小鳞茎可在MS+NAA0.2mg/l+6BA2mg/l(pH5.8——6.0)同一培养基上一次完成。接种后9天左右即可获得小鳞茎,一个月左右即可诱导形成完整植株。通过外部形态观察和细胞组织学研究表明小鳞茎的发生与不定芽的发生过程相类似,并讨论了小鳞茎发生过程中的极性现象,细胞起动形成愈伤组织的部位,诱导小鳞茎发生过程中淀粉消化的生理意义以及光对小鳞茎发生的作用等问题。

STUDY ON MORPHOGENESIS OF BULBLETS FROM BULB SCALES OF LILIUM DAVIDII VAR.UNICOLOR COTTON IN VITRO

The bulb-scales of Liliurn used as explantswere cultured on MS basic medium supplemented with NAA 0.2rag/l and 6-BA 2mg/l. At 9-10 days of culturing bulblets grew out from adaxial surface of the bulb-scales at the morphological lower end of the explants. It was also observed that the regenerative efficiency of the apical, middle or basal segment of a bulb-scale was similar.plantlets were obtained by induction of the roots on the same medium about after a month in culture. Under cytohistological observations on bulblet formation it was found that cell divisions began in the epidermal or subepidermal cells and the process of morphogenesis of bulblets was similar to that of adventitious buds. The starch grains in the tissue were utilized as available source of energy for the development of bulblets. Light may not be necessary for bulblet morphogenesis.