13C NMR structural investigation of scleroglucan |
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Authors: | Marguerite Rinaudo Marc Vincendon |
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Affiliation: | Centre de Recherches sur les Macromolécules Végétales, Laboratoire Propre du CNRS Associé à l''Université Scientifique et Médicale de Grenoble, 53 X, 38041, Grenoble-Cédex, France;Laboratoires de Chimie, Département de Recherche Fondamentale, Centre d''Etudes Nucléaires, 85 X, 38041 Grenoble-Cédex, France |
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Abstract: | This paper concerns the 13C NMR signal assignment in the DMSO of a neutral polysaccharide, scleroglucan. The previously proposed chemical structure is confirmed. The 13C NMR spectrum shows that scleroglucan is a regular poly (A, B, C, D) type glucan. The relaxation times of the different series of carbon atoms demonstrate that a single, pendant glucose group is attached to each third monomer along the main chain of what is a β(1 → 3)-glucan. Partial acid hydrolysis gives a spectrum analogous to that of the β(1 → 3)-d-glucan, curdlan, and confirms the structure of the polymer backbone.In aqueous solution, no signal has been obtained due to the existence of a rigid, ordered conformation as demonstrated by optical rotation; in the presence of sodium hydroxide, a conformational transition is produced just as with curdlan. The conclusion is that the behaviour of scleroglucan in solution is similar to that of other β(1 → 3)-d-glucans even though it is more soluble. |
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