Localization of a Cell Adhesion Site on Collagen XIV (Undulin) |
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Authors: | Tobias Ehnis Walburga Dieterich Michael Bauer Detlef Schuppan |
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Institution: | Klinikum Benjamin Franklin, Department of Gastroenterology, Free University of Berlin, Hindenburgdamm 30, D-12200, Berlin, Germany |
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Abstract: | Cell adhesion to collagen XIV is implied to be mediated by proteoglycans as cellular receptors (T. Ehniset al.,1996,Exp. Cell Res.229, 388–397). In order to define the cell binding region(s), fusion proteins expressed inEscherichia coliand covering the large noncollagenous domain NC3 of collagen XIV were used as substrates for the adhesion of skin fibroblasts. A prominent cell binding site could be localized in the N-terminal fibronectin type III repeat of collagen XIV and its immediate C-terminal extension. Since this region also mediates the binding of the small chondroitin/dermatan sulfate proteoglycan decorin (T. Ehniset al.,1997,J. Biol. Chem.272, 20414–20419), our finding could provide the molecular basis for the observation that decorin serves as inhibitor and potential modulator of cellular interactions with collagen XIV. |
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Keywords: | collagen XIV cell adhesion matrix receptor CD44 decorin proteoglycan |
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