Histochemical demonstration of hyaluronic acid molecules by Alcian Blue |
| |
| Authors: | E Reale L Luciano and M Spitznas |
| |
| Institution: | (1) Jules Stein Eye Institute, UCLA School of Medicine, University of California, Los Angeles, California, USA;(2) Present address: Laboratory of Electron Microscopy, Hannover Medical School, 3 Hannover 61, Federal Republic of Germany;(3) Present address: University Eye Hospital, University of Bonn, 53 Bonn, Federal Republic of Germany |
| |
| Abstract: | Summary Specimens of vitreous humour (monkey eye), Wharton jelly (human umbilical cord) and commercial hyaluronates were immersed in buffered fixative solutions containing either aldehydes and Alcian Blue, or aldehydes and Alcian Blue with MgCl2 as electrolyte. Two MgCl2 concentrations were used, 0.025m and 0.3m. Immersion in both solutions induced formation of precipitates which were postfixed in OsO4, dehydrated and embedded for thin section electron microscopy. The use of the same fixative solution produced morphologically comparable precipitates from all three materials. The precipitates, especially after fixation in the presence of electrolyte, were composed of linear, unbranched filaments, frequently aggregated into bundles. The filaments were considered to be molecules of hyaluronic acid.Part of this work was presented at the 10th Meeting of the European Club for Ophthalmic Fine Structure, Copenhagen, September 3–4, 1982. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
