Physical assignment of six type I anchor loci to bovine chromosome 19 by fluorescence in situ hybridization |
| |
Authors: | D. S. GallagherJr,Y.-P. Yang,J. D. Burzlaff,J. E. Womack,D. M. Stelly,S. K. Davis,& J. F. Taylor |
| |
Affiliation: | Department of Animal Science,;Department of Veterinary Pathobiology,;Department of Soil and Crop Sciences, Texas A&M University, College Station, Texas 77843, USA |
| |
Abstract: | A bovine bacterial artificial chromosome (BAC) library was screened for the presence of eight type I anchor loci previously used within hybrid somatic cells and an interspecies hybrid backcross to construct a genome map of bovine chromosome 19 (BTA19). Six out of eight loci were identified in the BAC library ( NF1, CRYB1, CHRNB1, TP53, GH1 and P4HB ). The BACs were then used in single-colour fluorescence in situ hybridization (FISH) to assign these genes to BTA19 band locations. Gene order was determined by single-colour FISH, and was confirmed by dual-colour FISH to mitotic and meiotic chromosomes. The order, centromere- NF1-CRYB1-CHRNB1-TP53-GH1-P4HB , was in agreement with the order determined by linkage analyses. In addition, the order of CHRNB1 and TP53 , previously unresolved by linkage analyses, was established. These data provide high-resolution cytogenetic anchorage of the BTA19 genome map from chromosome bands 14–22. |
| |
Keywords: | bovine cattle fluorescence in situ hybridization chromosomes physical gene mapping bovine bacterial artificial chromosome library |
|
|