POSTNATAL CHANGES IN THE POTASSIUM-STIMULATED, CALCIUM-DEPENDENT RELEASE OF RADIOACTIVE GABA AND GLYCINE FROM SLICES OF RAT CENTRAL NERVOUS TISSUE |
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| Authors: | L P Davies G A R Johnston A L Stephanson |
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| Institution: | Department of Pharmacology, John Curtin School of Medical Research, Australian National University, Canberra, Australia |
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| Abstract: | —Using a simple apparatus designed to perfuse nervous tissue mini-slices retained on glass fibre filter discs, slices of adult (13 week) rat cerebral cortex and spinal cord were shown to release radioactive GABA and glycine, but not 2-amino-isobutyric acid, in response to increased potassium ion concentration of the perfusing medium. A major portion of this potassium-stimulated release was dependent upon the presence of calcium ions in the perfusing medium. Slices of cerebral cortex and spinal cord from rats of 1 day and 10 days postnatal age showed potassium-stimulated, calcium-dependent release of radioactive GABA and glycine respectively. These findings are consistent with other evidence that GABA and glycine are functioning as inhibitory transmitters in rats at least as soon as 1 day after birth. |
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