|
|||||
|
|
Purification of recombinant laccase from Trametes versicolor in Pichia methanolica and its use for the decolorization of anthraquinone dye |
|
| Authors: | Mei Guo Fuping Lu Minyao Liu Tuoping Li Jun Pu Na Wang Peng Liang Chenyun Zhang |
| Institution: | (1) Department of Food Science, Tianjin Agriculture College, Tianjin, 300384, People’s Republic of China;(2) Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300222, People’s Republic of China;(3) Tianjin Nuoao Technology Company, Tianjin, 300384, People’s Republic of China |
| Abstract: | A recombinant laccase from Trametes versicolor in Pichia methanolica was produced constitutively in a defined medium. The recombinant laccase was purified using ultrafiltration, anion-exchange chromatography, and gel filtration. The molecular weight of the purified laccase was estimated as 64 kDa by SDS-PAGE. The purified recombinant laccase decolorized more than 90% of Remazol Brilliant Blue R (RBBR) initially at 80 mg l−1 after 16 h at 45°C and pH 5 when 25 U laccase ml−1 was used. The purified recombinant laccase could efficiently decolorize RBBR without additional redox mediators. |
| Keywords: | Decolorization Pichia methanolica Purification Recombinant laccase Remazol Brilliant Blue R |
| 本文献已被 PubMed SpringerLink 等数据库收录！ | |