Involvement of endopeptidase 24.15 in the inactivation of bradykinin by rat brain slices |
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| Authors: | J R McDermott A M Gibson J D Turner |
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| Affiliation: | 1. Department of Medical Laboratories, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi Arabia;2. 428 Church Street, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, United States of America;3. Department of Medical Laboratory Sciences, College of Health Sciences, University of Sharjah, University City, Sharjah 27272, United Arab Emirates;4. Deanship of Scientific Research, King Abdulaziz University, Jeddah, Saudi Arabia;5. Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 52571, Saudi Arabia;6. Department of Pathology, College of Medicine, Qassim University, P.O. Box 6655, Buraidah 51452, Saudi Arabia;7. College of Pharmacy and Health Sciences, Ajman University, United Arab Emirates;8. Centre of Medical and Bio-allied Health Sciences Research, Ajman University, United Arab Emirates;9. Department of Pharmacognosy, College of Pharmacy, King Khalid University (KKU), Guraiger St., Abha 62529, Saudi Arabia;10. Complementary and Alternative Medicine Unit, King Khalid University (KKU), Guraiger St., Abha 62529, Saudi Arabia;1. Department of Chemistry, Faculty of Mathematical and Natural Sciences, Pristina University, 10000, Republic of Kosova, Albania;2. Department of Chemistry, Faculty of Arts and Science, Sakarya University, 54187, Sakarya, Turkey;3. Department of Biochemistry, Faculty of Pharmacy, Erzincan Binali Yıldırım University, 24002 Erzincan, Turkey;4. Department of Pharmacy Services, Nihat Delibalta Göle Vocational High School, Ardahan University, 75700 Ardahan, Turkey;5. Department of Pharmacology, Faculty of Pharmacy, Erzincan Binali Yıldırım University, 24002 Erzincan, Turkey;6. Department of Biochemistry, Faculty of Pharmacy, Anadolu University, 26470 Eskişehir, Turkey;7. Bilecik Şeyh Edebali University, 11230 Bilecik, Turkey |
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| Abstract: | The effect of peptidase inhibitors on the degradation of [3H]-bradykinin by rat hypothalamic slices was studied using HPLC to separate and identify the products. The degradation appears to be mainly mediated by an enzyme which cleaves the peptide at the Phe5-Ser6 bond and is inhibited by 1,10-phenanthroline, dynorphin(1-13) and carboxyphenylethyl-Ala-Ala-Phe-p-aminobenzoate. This suggest the involvement of a membrane bound variant of the soluble metalloendopeptidase (EC3.4.24.15) isolated from rat brain which degrades neurotensin, angiotensin and other neuropeptides as well as bradykinin. |
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