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Determination of standard zinc values in the intact tissues of mice by ICP spectrometry
Authors:Donatella Verbanac  Cedomila Milin  Robert Domitrovic  Jasminka Giacometti  Radojka Pantovic  Zlatko Ciganj
Institution:(1) Medical Faculty, Department of Chemistry and Biochemistry, University of Rijeka, Braće Branchetta 20, HR 51000 Rijeka, Croatia;(2) INA-Refinery Urinj, HR 51000 Rijeka, Croatia
Abstract:The most commonly encountered difficulties for the quantitative measurement of zinc in biological samples are the limited sample amount, total and effective digestion of connective and fatty residues, and low zinc concentrations. These problems often lead to the determination of lower zinc values than actually present, so that the sample preparation, digestion, and analytical procedure deserve careful attention. In this short communication, a new method for microwave tissue disintegration is described. The authors have obtained consistent and reproducible results with tissue samples of 0.5 g or less.
Keywords:Zinc  microwave digestion  ICP spectroscopy  atomic absorption  zinc in tissue  zinc in serum
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