Benzyladenine-controlled protein synthesis and growth in apple cell suspensions |
| |
Authors: | Ricardo J Ordás Belen Fernández Roberto Rodríguez |
| |
Institution: | Departamento B. O. S., Facultad de Biologia, Univ. de Oviedo, E-33005 Oviedo, Spain. |
| |
Abstract: | Prolific growth in cytokinin-requiring apple cell suspensions was achieved during 10 days of culture with appropriate concentrations of benzyladenine (BA). Unlike the controls, BA-treated cells showed a well developed sub-cellular organization and protein synthesis. Upon transfer to fresh cytokinin-free medium, however, these cells exhibited a rapid decrease in polyribosome formation, but with unchanged nuclear and nucleolar activities. Cells lacking added cytokinin showed a reduced metabolic activity and failed to divide. Deficiency in the translation process of cytokinin-deprived cells was overcome by exogenous BA-treatment. In cells maintained on a cytokinin-free medium for 5 days, BA-treatment enhanced protein synthesis from pre-existing mRNA in a specific way. |
| |
Keywords: | Apple cell suspension cytokinin Malus domestica translation |
|
|